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eea1 anti rabbit antibody  (Proteintech)


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    Proteintech eea1 anti rabbit antibody
    Differences in viral internalization of the HuN4-F112 virus and its mutants in PAM cells. ( A ) Confocal microscopy analysis of viral internalization. The parental HuN4-F112 and mutant viruses were inoculated into PAM cells at an MOI of 50. The internalization of the virus on PAM cells was imaged, and colocalization with <t>EEA1</t> was detected. ( B ) Quantitative analysis of the internalized virus. The parental rHuN4-F112 and mutant viruses were inoculated into PAM cells at an MOI of 50. After attachment at 4°C for 1 h, the cells were washed with cold PBS, and the amount of attached virus was measured via RT-qPCR. After incubation at 37°C for 2 h, the cells were washed eight times with cold PBS at pH 1.3, and the amount of internalized virus was measured via RT-qPCR. ( C ) Ratio of internalized virus to attached virus. ns, not significant. ***, P < 0.001; ****, P < 0.0001.
    Eea1 Anti Rabbit Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eea1 anti rabbit antibody/product/Proteintech
    Average 95 stars, based on 100 article reviews
    eea1 anti rabbit antibody - by Bioz Stars, 2026-03
    95/100 stars

    Images

    1) Product Images from "GP2a I118 and GP4 D43 play critical roles in the attachment of PRRSV to the CD163 receptor: implications for anti-PRRSV infection targets"

    Article Title: GP2a I118 and GP4 D43 play critical roles in the attachment of PRRSV to the CD163 receptor: implications for anti-PRRSV infection targets

    Journal: Journal of Virology

    doi: 10.1128/jvi.00963-25

    Differences in viral internalization of the HuN4-F112 virus and its mutants in PAM cells. ( A ) Confocal microscopy analysis of viral internalization. The parental HuN4-F112 and mutant viruses were inoculated into PAM cells at an MOI of 50. The internalization of the virus on PAM cells was imaged, and colocalization with EEA1 was detected. ( B ) Quantitative analysis of the internalized virus. The parental rHuN4-F112 and mutant viruses were inoculated into PAM cells at an MOI of 50. After attachment at 4°C for 1 h, the cells were washed with cold PBS, and the amount of attached virus was measured via RT-qPCR. After incubation at 37°C for 2 h, the cells were washed eight times with cold PBS at pH 1.3, and the amount of internalized virus was measured via RT-qPCR. ( C ) Ratio of internalized virus to attached virus. ns, not significant. ***, P < 0.001; ****, P < 0.0001.
    Figure Legend Snippet: Differences in viral internalization of the HuN4-F112 virus and its mutants in PAM cells. ( A ) Confocal microscopy analysis of viral internalization. The parental HuN4-F112 and mutant viruses were inoculated into PAM cells at an MOI of 50. The internalization of the virus on PAM cells was imaged, and colocalization with EEA1 was detected. ( B ) Quantitative analysis of the internalized virus. The parental rHuN4-F112 and mutant viruses were inoculated into PAM cells at an MOI of 50. After attachment at 4°C for 1 h, the cells were washed with cold PBS, and the amount of attached virus was measured via RT-qPCR. After incubation at 37°C for 2 h, the cells were washed eight times with cold PBS at pH 1.3, and the amount of internalized virus was measured via RT-qPCR. ( C ) Ratio of internalized virus to attached virus. ns, not significant. ***, P < 0.001; ****, P < 0.0001.

    Techniques Used: Virus, Confocal Microscopy, Mutagenesis, Quantitative RT-PCR, Incubation



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    Differences in viral internalization of the HuN4-F112 virus and its mutants in PAM cells. ( A ) Confocal microscopy analysis of viral internalization. The parental HuN4-F112 and mutant viruses were inoculated into PAM cells at an MOI of 50. The internalization of the virus on PAM cells was imaged, and colocalization with <t>EEA1</t> was detected. ( B ) Quantitative analysis of the internalized virus. The parental rHuN4-F112 and mutant viruses were inoculated into PAM cells at an MOI of 50. After attachment at 4°C for 1 h, the cells were washed with cold PBS, and the amount of attached virus was measured via RT-qPCR. After incubation at 37°C for 2 h, the cells were washed eight times with cold PBS at pH 1.3, and the amount of internalized virus was measured via RT-qPCR. ( C ) Ratio of internalized virus to attached virus. ns, not significant. ***, P < 0.001; ****, P < 0.0001.
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    Differences in viral internalization of the HuN4-F112 virus and its mutants in PAM cells. ( A ) Confocal microscopy analysis of viral internalization. The parental HuN4-F112 and mutant viruses were inoculated into PAM cells at an MOI of 50. The internalization of the virus on PAM cells was imaged, and colocalization with <t>EEA1</t> was detected. ( B ) Quantitative analysis of the internalized virus. The parental rHuN4-F112 and mutant viruses were inoculated into PAM cells at an MOI of 50. After attachment at 4°C for 1 h, the cells were washed with cold PBS, and the amount of attached virus was measured via RT-qPCR. After incubation at 37°C for 2 h, the cells were washed eight times with cold PBS at pH 1.3, and the amount of internalized virus was measured via RT-qPCR. ( C ) Ratio of internalized virus to attached virus. ns, not significant. ***, P < 0.001; ****, P < 0.0001.
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    Differences in viral internalization of the HuN4-F112 virus and its mutants in PAM cells. ( A ) Confocal microscopy analysis of viral internalization. The parental HuN4-F112 and mutant viruses were inoculated into PAM cells at an MOI of 50. The internalization of the virus on PAM cells was imaged, and colocalization with <t>EEA1</t> was detected. ( B ) Quantitative analysis of the internalized virus. The parental rHuN4-F112 and mutant viruses were inoculated into PAM cells at an MOI of 50. After attachment at 4°C for 1 h, the cells were washed with cold PBS, and the amount of attached virus was measured via RT-qPCR. After incubation at 37°C for 2 h, the cells were washed eight times with cold PBS at pH 1.3, and the amount of internalized virus was measured via RT-qPCR. ( C ) Ratio of internalized virus to attached virus. ns, not significant. ***, P < 0.001; ****, P < 0.0001.
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    Image Search Results


    Differences in viral internalization of the HuN4-F112 virus and its mutants in PAM cells. ( A ) Confocal microscopy analysis of viral internalization. The parental HuN4-F112 and mutant viruses were inoculated into PAM cells at an MOI of 50. The internalization of the virus on PAM cells was imaged, and colocalization with EEA1 was detected. ( B ) Quantitative analysis of the internalized virus. The parental rHuN4-F112 and mutant viruses were inoculated into PAM cells at an MOI of 50. After attachment at 4°C for 1 h, the cells were washed with cold PBS, and the amount of attached virus was measured via RT-qPCR. After incubation at 37°C for 2 h, the cells were washed eight times with cold PBS at pH 1.3, and the amount of internalized virus was measured via RT-qPCR. ( C ) Ratio of internalized virus to attached virus. ns, not significant. ***, P < 0.001; ****, P < 0.0001.

    Journal: Journal of Virology

    Article Title: GP2a I118 and GP4 D43 play critical roles in the attachment of PRRSV to the CD163 receptor: implications for anti-PRRSV infection targets

    doi: 10.1128/jvi.00963-25

    Figure Lengend Snippet: Differences in viral internalization of the HuN4-F112 virus and its mutants in PAM cells. ( A ) Confocal microscopy analysis of viral internalization. The parental HuN4-F112 and mutant viruses were inoculated into PAM cells at an MOI of 50. The internalization of the virus on PAM cells was imaged, and colocalization with EEA1 was detected. ( B ) Quantitative analysis of the internalized virus. The parental rHuN4-F112 and mutant viruses were inoculated into PAM cells at an MOI of 50. After attachment at 4°C for 1 h, the cells were washed with cold PBS, and the amount of attached virus was measured via RT-qPCR. After incubation at 37°C for 2 h, the cells were washed eight times with cold PBS at pH 1.3, and the amount of internalized virus was measured via RT-qPCR. ( C ) Ratio of internalized virus to attached virus. ns, not significant. ***, P < 0.001; ****, P < 0.0001.

    Article Snippet: The cells were incubated with PRRSV N monoclonal antibody (1B4) and EEA1 anti-rabbit antibody (Proteintech, China) overnight at 4°C; after being washed with PBS, anti-mouse IgG-FITC antibody (Sigma, USA) and anti-rabbit IgG-Cy3 antibody (ABclonal, China) were added for 1 h, and then the cells were stained with DAPI for 15 min.

    Techniques: Virus, Confocal Microscopy, Mutagenesis, Quantitative RT-PCR, Incubation